Assessment of the cytotoxic activity of a compound on a model of the human alveolar tissue
Human and animal health In vitro/ex vivo cytotoxicity
Background of in vitro - 3D tissue model development
- Cell culture is an important and necessary process in drug discovery and cancer research
- Two-dimensional (2D) cell culture has been the method used to culture cells since the early 1900s but has many limitations due to 2D models inaccurately representing tissue cells in vitro
- 3D tissue model of the human airways EpiAlveolar (MatTek) was engineered to provide human-relevant results in an easy-to-use in vitro system
- EpiAlveolar tissues are produced on cell culture inserts. The epithelium exhibits functional tight junctions and a robust barrier
Objectives of the model
- To determine the putative cytotoxic activity of a molecule using a 3D tissue model of the human airways
Our approach at Vibiosphen
- A range of concentrations of the molecule was applied on the tissue in triplicate
- The plates were incubated at 37°C 5% CO2
- Viable cells were quantified with Cell Titer Glo 2.0 cell viability assay
Outcomes of in vitro - 3D tissue model
- 3D tissue models originated from human cells provide human-relevant results and therefore contribute to avoiding the late-stage failures of in vivo testing
- 3D tissue models can be used in drug discovery, cancer research….
Why Choose Vibiosphen?
- Proven expertise in preclinical research
- Flexible and customized study designs adapted to sponsor requirements (see downloads available as example)
- Advanced facilities to ensure reliable and reproducible results (BSL2 and BSL3)
- Strong collaborations with pharmaceutical companies, biotech firms, and academic partners
Vibiosphen combines scientific excellence with industry know-how to deliver actionable preclinical data that drive innovation in infectious disease therapeutics.
Contact Us
- Contact us today to discuss your project and explore how our models can support your development strategy.
- We will be pleased to facilitate your project by providing a customized study design to your project objectives.