Reconstructed Human Epidermis (RHE)
Ex vivo 3D tissue
Background of Reconstructed Human Epidermis (RHE)
The Reconstructed Human Epidermis (RHE) is a 3D tissue model grown from human keratinocytes that is histologically similar to the in vivo human epidermis, including the formation of a stratum corneum (the outermost barrier layer).
RHE model offers numerous significant advantages for research and testing in the field of dermocosmetics. These benefits address both ethical concerns and scientific rigor.
RHE are validated and used for assessing both the safety and efficacy of cosmetic ingredients and finished products.
Objectives of the model Reconstructed Human Epidermis (RHE)
• Safety Assessment. Skin Irritation and Corrosivity: RHE models have replaced animal testing for assessing skin irritation potential (OECD Test Guideline 439). They allow for the measurement of endpoints like cell viability and pro-inflammatory mediators. Phototoxicity: RHE are used to assess the phototoxic potential of chemicals and cosmetics (e.g., cell viability after UV exposure).
• Efficacy & Claim Substantiation. UV Response: Ideal for studies on the effects of UV protection products and studying photoaging. Molecular and 'Omics' Studies: Suitable for analyzing gene expression, biomarker collection, and omics (genomics, proteomics) to substantiate claims related to anti-aging, moisturizing, and anti-inflammatory effects at a molecular level.
• RHE is a powerful platform for skin microbiota research. RHE can be co-cultured with a selection of microbial strains representative of the skin's micro-environments to advance the development of microbiome-targeted dermatological and cosmetic products
Our approach at Vibiosphen
The co-culture assay was used to address the fungal-host interactions, particularly relevant to dandruff pathology:
-Topical application of Malassezia restricta on Reconstructed Human Epidermis (RHE)
-Histological assessment of epidermal morphology
-Quantification of specific biomarkers related to skin function
-Quantification of M. restricta colonization
Outcomes of Reconstructed Human Epidermis (RHE)
This model was used to investigate novel anti-dandruff treatments aimed at blocking M. restricta-mediated lipoperoxidation and restoring epidermal homeostasis.