Assessment of the cytotoxic activity of a compound on a model of the human alveolar tissue

Background of in vitro- 3D tissue model development

•    Cell culture is an important and necessary process in drug discovery and cancer research
•    Two-dimensional (2D) cell culture has been the method used to culture cells since the early 1900s but has many limitations due to 2D models inaccurately representing tissue cells in vitro
•    3D tissue model of the human airways EpiAlveolar (MatTek) was engineered to provide human-relevant results in an easy-to-use in vitro system
•    EpiAlveolar tissues are produced on cell culture inserts. The epithelium exhibits functional tight junctions and a robust barrier

Objectives of the model

•    To determine the putative cytotoxic activity of a molecule using a 3D tissue model of the human airways

Our approach at Vibiosphen 

•    A range of concentrations of the molecule was applied on the tissue in triplicate
•    The plates were incubated at 37°C 5% CO2
•    Viable cells were quantified with Cell Titer Glo 2.0 cell viability assay

Outcomes of in vitro- 3D tissue model

•    3D tissue models originated from human cells provide human-relevant results and therefore contribute to avoiding the late-stage failures of in vivo testing
•    3D tissue models can be used in drug discovery, cancer research….

Why Choose Vibiosphen?

•    Proven expertise in preclinical research
•    Flexible and customized study designs adapted to sponsor requirements (see downloads available as example) 
•    Advanced facilities to ensure reliable and reproducible results (BSL2 and BSL3) 
•    Strong collaborations with pharmaceutical companies, biotech firms, and academic partners
Vibiosphen combines scientific excellence with industry know-how to deliver actionable preclinical data that drive innovation in infectious disease therapeutics.
 

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If you are developing new treatments or vaccines, Vibiosphen can help accelerate your research.
Contact us today to discuss your project and explore how our Pharmacokinetics models can support your development strategy. 
We will be pleased to facilitate your project by providing a customized study design to your project objectives.