Determination of the resistance development against an antibacterial compound

Background of in vitro-resistance development

•    The propensity for the development of bacterial resistance to conventional antibiotics poses serious public health problems

•    The development of long-lasting antibacterial agents is in high demand to overcome the complications of antimicrobial resistance.
•    Quantifying the frequency of resistance development is valuable information for the development of new antibacterial drugs.

Objectives of the model

•    To quantify the increased resistance to reference antibiotics of two bacterial species, subsequently to successive growth in the presence of increasing amount of the antibiotics.

Our approach at Vibiosphen 

•    The Minimal Inhibitory Concentration (MIC) of reference antibiotics meropenem and tigecycline against Pseudomonas aeruginosa and Acinetobacter baumannii were determined
•    P. aeruginosa and A. baumannii were grown in broth in the presence of sub-MIC concentrations of meropenem and tigecyline, respectively, for 11 days.
•    MIC were measured every other day.

Outcomes of in vitro-resistance development

The fold of increase in MIC for the antibiotics were plotted against the number of passages (days).

Susceptibility towards bacterial resistance development: 
Comparisons of fold increase in MIC

•    The overall result showed that this experimental approach can reveal the tendency for the development of bacterial resistance to a new antibacterial drug.

Why Choose Vibiosphen?

•    Proven expertise in preclinical research
•    Flexible and customized study designs adapted to sponsor requirements (see downloads available as example) 
•    Advanced facilities to ensure reliable and reproducible results (BSL2 and BSL3) 
•    Strong collaborations with pharmaceutical companies, biotech firms, and academic partners
Vibiosphen combines scientific excellence with industry know-how to deliver actionable preclinical data that drive innovation in infectious disease therapeutics.
 

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