Background of Minimum bactericidal concentration (MBC)

The Minimum Bactericidal Concentration (MBC) is the lowest concentration of an antimicrobial agent needed to kill at least 99.9% of a specific bacterial population after a defined period of incubation.
While the Minimum Inhibitory Concentration (MIC) is the minimum concentration that prevents visible growth, the MBC is the minimum concentration needed to achieve a lethal effect.

Background of Minimum inhibitory concentration (MIC)

The minimum inhibitory concentration (MIC) is the lowest concentration of an antimicrobial that prevents visible growth of a microorganism. 

Determining the MIC for any new antimicrobial is a fundamental step in its development:

Background of the quantification of intracellular bacteria 

Background of time kill curve assay (TKC)