Background of Minimum Bactericidal Concentration (MBC)

The Minimum Bactericidal Concentration (MBC) is the lowest concentration of an antimicrobial agent needed to kill at least 99.9% of a specific bacterial population after a defined period of incubation.
While the Minimum Inhibitory Concentration (MIC) is the minimum concentration that prevents visible growth, the MBC is the minimum concentration needed to achieve a lethal effect.

Background of Minimum Inhibitory Concentration (MIC)

The Minimum Inhibitory Concentration (MIC) is the lowest concentration of an antimicrobial that prevents visible growth of a microorganism. 

Determining the MIC for any new antimicrobial is a fundamental step in its development:

Background of Model of in vitro human scalp microbiota

• Based on 454 sequencing of the scalp microbiome, the two most abundant bacterial genera found on the scalp surface are Staphylococcus and Cutibacterium, while Malassezia is the main fungal inhabitant (Grimshaw et al., 2019).
• The human scalp hosts an unusually low diversity microbiota dominated by three species: S. epidermidis, C. acnes and M.